14 April (Monday)

• 08:30 - 18:30

  Registration
• 09:00 - 09:05

  Welcome

  Malda Jos (NL)
• 09:05 - 09:30

  Defining the Field: Joint Preservation from Bedside to Bench and Back

  Custers Roel (NL), Van Egmond Nienke (NL)
• 09:30 - 10:15

  My Journey in Cartilage Regeneration and Joint Preservation

  Grande Daniel (US)
• 10:15 - 10:45

  Break + Walk to Labspace
• 10:45 - 11:00

  The Concepts of Chondrocyte Isolation

  Van Osch Gerjo (NL)
• 11:00 - 12:00

  Chondrocyte Isolation in Practice (Hands-on/Lab)

  Korpershoek Jasmijn (NL)
• 12:00 - 12:15

  Short Break / Unforeseen Lab Time
• 12:15 - 13:15

  How to Make Clinical Impact with Translational and Fundamental Research (1+1=3)

  Saris Daniel (US), Vonk Lucienne (DE)
• 13:15 - 14:15

  Lunch Break
• 14:15 - 16:15

  Chondrocyte Seeding in Monolayers, 3D Scaffolds and Bio-inks (Hands-on/Lab)

  Korpershoek Jasmijn (NL)
• 16:15 - 16:30

  Short Break
• 16:30 - 17:00

  The Secrets of the Cartilage ECM

  Zaucke Frank (DE)
• 17:30 - 19:30

  Move to City / Welcome Reception

15 April (Tuesday)

• 08:30 - 09:00

  Introduction into Bioprinting of Cartilage and 3D Surgical Planning

  Malda Jos (NL)
• 09:00 - 09:30

  Bioprinting Technologies

  Levato Riccardo (NL)
• 09:30 - 11:00

  Bioprint Cells (Small Group Demos) (Hands-on/Lab)

  De Ruijter Mylene (NL), Nguyen H. Chien (NL)

  • Perform printability assay with Pluronics and cell-laden gel
  • Print on top of MEW mesh/ plug
  • 3D lab
  • Transfer plugs to bioreactor system and start system
• 11:00 - 11:30

  Break
• 11:30 - 12:00

  In Vitro Models of Cartilage

  Ito Keita (NL)
• 12:00 - 13:30

  On Chip Systems (Hands-on/Lab)

  De Ruijter Mylene (NL)

  • LIFT demo, Stirr and pour PDMS in chip mold
  • Plasma bind chip on glass slide
  • Connect and perfuse chip

     
• 13:30 - 14:30

  Lunch Break
• 14:30 - 15:00

  Bone Quantification Using Micro CT Imaging

  Rios Jaqueline Lourdes (NL), Weinans Harrie (NL)
• 15:00 - 16:00

  Cartilage and Bone Assessment (Histology and Scoring of Cartilage and Bone) (Hands-on/Lab)

  van Rijen Mattie (NL)
• 16:00 - 17:00

  Bone Quantification (micro CT) Potentially with 3D Lab (Hands-on/Lab)

  Warmink Kelly (NL), Weinans Harrie (NL)
• 19:00 - 22:30

  Course Dinner

16 April (Wednesday)

• 08:30 - 10:30

  In Vivo (large animal) Models
  • 30 min

    Horse as Translational Model

    Van Weeren René (NL)
  • 30 min

    Species Differences

    Malda Jos (NL)
  • 30 min

    Demo Gait and Tour and Tour of the Equine Facilities

    Brommer Harold (NL)
  • 30 min

    Demo OC Defect Model Preparation in the Wetlab

    De Ruijter Mylene (NL), Malda Jos (NL)
• 10:30 - 11:00

  Break
• 11:00 - 12:00

  Back to the Clinic (Video surgery) / Wrap up

  Custers Roel (NL), Van Egmond Nienke (NL)